Recombinant Mouse FLRT2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10715-FL

R&D Systems, part of Bio-Techne
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10715-FL-050
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Key Product Details

Source

NS0

Accession #

Q8BLU0.1

Conjugate

Unconjugated

Applications

Bioactivity

View all FLRT2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FLRT2 protein
Cys36-Ser539, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys36

Predicted Molecular Mass

57 kDa

SDS-PAGE

71-80 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
The ED50 for this effect is 0.60-4.80 µg/mL.

Scientific Data Images for Recombinant Mouse FLRT2 His-tag Protein, CF

Recombinant Mouse FLRT2 His-tag Protein Bioactivity.

Recombinant Mouse FLRT2 His-tag (Catalog # 10715-FL) supports the adhesion of Neuro 2A mouse neuroblastoma cells. The ED50 for this effect is 0.60-4.80 μg/mL.

Recombinant Mouse FLRT2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse FLRT2 His-tag (Catalog # 10715-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-80 kDa.

Formulation, Preparation and Storage

10715-FL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLRT2

FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) mouse FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa TM segment and a 99 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The mouse FLRT2 ECD shares 97% and 99% aa sequence identity with human and rat FLRT2 ECD, respectively. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2). Overexpression of FLRT2 promotes tumor suppression in colorectal and breast cancers (6, 7).

References

  1. Lacy, S. E. et al. (1999) Genomics 62:417.
  2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  6. Guo, X. et al. (2020) J. Cancer 11:7329.
  7. Bae, H. et al. (2017) Sci. Rep. 7:272.

Long Name

Fibronectin Leucine Rich Transmembrane Protein 2

Alternate Names

Fibronectin Leucine Rich Transmembrane Protein 2, Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2, KIAA0405, Leucine-Rich Repeat Transmembrane Protein FLRT2

Entrez Gene IDs

23768 (Human); 399558 (Mouse)

Gene Symbol

FLRT2

UniProt

Q8BLU0.1

Additional FLRT2 Products

Product Documents for Recombinant Mouse FLRT2 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse FLRT2 His-tag Protein, CF

For research use only

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