Recombinant Mouse FOLR1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6936-FR

R&D Systems, part of Bio-Techne
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6936-FR-050
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Key Product Details

Source

CHO

Accession #

NP_032060

Conjugate

Unconjugated

Applications

Bioactivity

View all FOLR1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse FOLR1 protein
Met1-Ser232, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg23

Predicted Molecular Mass

25.3 kDa

SDS-PAGE

29-36 kDa, reducing conditions

Activity

Measured in a competitive binding assay.

When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rmFOLR1 (7.9 nM) at the concentration range of 30-120 nM.

Formulation, Preparation and Storage

6936-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 7). Mouse FOLR1 shares 83% and 93% amino acid sequence identity with human and rat FOLR1, respectively. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas (8, 9). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (10). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 11). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (12).

References

  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Brigle, K.E. et al. (1991) J. Biol. Chem. 266:17243.
  8. Ross, J.F. et al. (1994) Cancer 73:2432.
  9. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  10. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  11. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  12. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Alternate Names

FBP, Folbp1, FR-alpha, MOv18

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Gene Symbol

FOLR1

UniProt

NP_032060

Additional FOLR1 Products

Product Documents for Recombinant Mouse FOLR1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse FOLR1 Protein, CF

For research use only

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