Recombinant Mouse Glypican 6 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9429-GP

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q9R087

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Glypican 6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Glypican 6 protein
Mouse Glypican 6
(Asp24-Val513)
Accession # Q9R087		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp24 & Ser355

Predicted Molecular Mass

83 kDa (Asp24-Val513/mFc), 44 kDa (Ser355-Val513/mFc) and 38 kDa (Asp24-Arg354)

SDS-PAGE

84-98 kDa (Asp24-Val513/mFc), 53-68 kDa (Asp24-Val513/mFc) and 33-40 kDa (Asp24-Arg354)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Glypican 6 Fc Chimera is immobilized at 3 μg/mL, 100 μL/well, the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) that produces 50% of the optimal binding response is 2.5-15 ng/mL.

Scientific Data Images for Recombinant Mouse Glypican 6 Fc Chimera Protein, CF

Recombinant Mouse Glypican 6 Fc Chimera Protein Bioactivity

Recombinant Mouse Glypican 6 Fc Chimera Protein Bioactivity

When Recombinant Mouse Glypican 6 Fc Chimera (Catalog # 9429-GP) is immobilized at 3 µg/mL, 100 µL/well, Recombinant Human FGF basic 146 aa (Catalog # 233-FB) binds with an ED50 of 2.5-15 ng/mL.

Formulation, Preparation and Storage

9429-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 6

The Glypicans (Glypiated Proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60-70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Mouse Glypican 6 (GPC-6) is synthesized as a 555 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 507 aa mature region and a 25 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan. Mouse to human, there is 96% aa identity over the entire GPC-6 molecule (5). Glypican proteins are located in the extracellular matrix of cells and are important for tissue organization, cell proliferation, adhesion, migration and differentiation when they interact with extracellular or cytoplasmic ligands (7, 8). Glypicans act as co‑receptors to enhance the activity of heparin-binding factors such as fibroblast growth factors and Wnt. Due to their ability to affect growth factor-mediated signaling, glypicans are known to have both pro- and anti-tumorigenic effects. GPC-6 overexpression has been associated with metastatic breast cancer, gastric cancer, and is known to regulate cardiomyocyte growth through the ERK1/2 signalling pathway where up-regulation is known to play a role in heart failure (9-11).

References

  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
  2. Filmus, J. (2001) Glycobiology 11:19R.
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  4. Filmus, J. (2003) Glycoconj. J. 19:319.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
  7. Kirn-Safran, C. et al. (2009) Cell. Mol. Life Sci. 66:3421.
  8. Syrokou, A. et al. (1999) Cell Prolif. 32:85.
  9. Dinccelik-Aslan, M. et al. (2015) Mol. Clin. Oncol. 3:584.
  10. Yiu, G.K. et al. (2011) Biochem. J. 440:157.
  11. Melleby, A.O. et al. (2016) PLoS One. 11:e0165079.

Alternate Names

GPC6

Entrez Gene IDs

10082 (Human); 23888 (Mouse)

Gene Symbol

GPC6

UniProt

Q9R087

Additional Glypican 6 Products

Product Documents for Recombinant Mouse Glypican 6 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Glypican 6 Fc Chimera Protein, CF

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