Recombinant Mouse HIN-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2954-HN

R&D Systems, part of Bio-Techne
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2954-HN-050
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Key Product Details

Source

E. coli

Accession #

NP_473378

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all HIN-1/SCGB3A1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse HIN-1/SCGB3A1 protein
Phe22-Gly104

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe22

Predicted Molecular Mass

8.4 kDa (monomer)

SDS-PAGE

6 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells.
The ED50 for this effect is 0.5-2.5 μg/mL.

Formulation, Preparation and Storage

2954-HN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIN-1/SCGB3A1

High in Normal-1 (HIN-1), also known as Uteroglobin related protein 2 (UGRP2), is an 8 kDa secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN-1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2-5). It is up‑regulated in Clara cells by IL-4, IL‑13, Oncostatin M, EGF, and TGF-alpha and down‑regulated by IFN-alpha, beta, and gamma (6-9). It is secreted into bronchial lavage fluid, saliva, and plasma and may form disulfide‑linked dimers (10). HIN-1 binds to the macrophage scavenger receptor MARCO and to the surface of mammary and bronchial epithelial cells (11, 12). HIN‑1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells likely through the Akt signaling pathway (12). The down‑regulation of HIN-1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 13, 10, 14). Mature mouse HIN-1 shares 58% and 93% aa sequence identity with human and rat HIN-1, respectively (4). A variant of mouse HIN-1 has a substitution at the N‑terminus in place of its potential signal peptide (5).

References

  1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
  2. Porter, D. et al. (2002) Mech. Dev. 114:201.
  3. Reynolds, S.D. et al. (2002) Am. J. Respir. Crit. Care Med. 166:1498.
  4. Krop, I. et al. (2001) Proc. Natl. Acad. Sci. 98:9796.
  5. Niimi, T. et al. (2002) Cytogenet. Genome Res. 97:120.
  6. Yamada, A. et al. (2005) J. Immunol. 175:5708.
  7. Tomita, T. et al. (2009) Am. J. Respir. Cell Mol. Biol. 40:620.
  8. Yamada, A. and S. Kimura (2005) FEBS Lett. 579:2221.
  9. Yamada, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:964.
  10. Krop, I. et al. (2004) Mol. Cancer Res. 2:489.
  11. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
  12. Krop, I. et al. (2005) Cancer Res. 65:9659.
  13. Krop, I. et al. (2003) Cancer Res. 63:2024.
  14. Shigematsu, H. et al. (2005) Int. J. Cancer 113:600.

Long Name

High in Normal 1

Alternate Names

HIN1, PnSP-2, SCGB3A1, UGRP2

Entrez Gene IDs

92304 (Human); 73945 (Mouse)

Gene Symbol

SCGB3A1

UniProt

NP_473378

Additional HIN-1/SCGB3A1 Products

Product Documents for Recombinant Mouse HIN-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse HIN-1 Protein, CF

For research use only

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