Recombinant Mouse ICOS Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 168-CS
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse ICOS protein
| Mouse ICOS (Glu21-Leu142) Accession # NP_059508 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu21
Predicted Molecular Mass
44 kDa (monomer)
SDS-PAGE
47 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized rmICOS/Fc Chimera at 1 µg/mL (100 µL/well) can bind biotinylated rhB7-H2/Fc Chimera with a linear range of 0.156-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Immobilized rmICOS/Fc Chimera at 1 µg/mL (100 µL/well) can bind biotinylated rhB7-H2/Fc Chimera with a linear range of 0.156-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
168-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICOS
Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Hutloff, A. et al. (1999) Nature 397:263.
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
Long Name
Inducible Co-stimulator
Alternate Names
AILIM, CD278, CRP-1
Gene Symbol
ICOS
UniProt
Additional ICOS Products
Product Documents for Recombinant Mouse ICOS Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse ICOS Fc Chimera Protein, CF
For research use only
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