Recombinant Mouse IFN-epsilon Protein
R&D Systems, part of Bio-Techne | Catalog # 9147-ME
Key Product Details
Product Specifications
Source
E. coli-derived mouse IFN-epsilon protein
Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag
Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag
Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
21 kDa
SDS-PAGE
20 kDa, reducing conditions
Activity
Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 6.00-60.0 ng/mL.
The ED50 for this effect is 6.00-60.0 ng/mL.
Scientific Data Images for Recombinant Mouse IFN-epsilon Protein
Recombinant Mouse IFN-epsilon Protein Bioactivity
Recombinant Mouse IFN-epsilon (Catalog # 9147-ME) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 6.00-60.0 ng/mL.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 9147-ME
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 9147-ME/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS |
| Reconstitution | Reconstitute at 100 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IFN-epsilon
(2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation and the attraction of CD8+ T cells to sites of virus infection (5, 7).
References
- Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
- Fung, K.Y. et al. (2013) Science 339:1088.
- Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
- Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
- Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
- Hermant, P. et al. (2013) PLoS One 8:e71320.
- Day, S.L. et al. (2008) J. Immunol. 180:7158.
Long Name
Interferon epsilon
Alternate Names
Ifn-tau-11, IFNE, IFNepsilon, IFNT1, INFE1
Gene Symbol
IFNE
UniProt
Additional IFN-epsilon Products
Product Documents for Recombinant Mouse IFN-epsilon Protein
Product Specific Notices for Recombinant Mouse IFN-epsilon Protein
For research use only
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