Recombinant Mouse IGFBP-4 Protein

R&D Systems, part of Bio-Techne | Catalog # 8066-GB

R&D Systems, part of Bio-Techne
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Carrier Free
8066-GB-025/CF
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With Carrier
8066-GB-025
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Key Product Details

Source

NS0

Accession #

P47879

Conjugate

Unconjugated

Applications

Bioactivity

View all IGFBP-4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IGFBP-4 protein
Asp22-Glu254

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp22

Predicted Molecular Mass

26 kDa

SDS-PAGE

30-36 kDa, reducing conditions

Activity

Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 0.02-0.12 μg/mL in the presence of 14 ng/mL of Recombinant Mouse IGF‑II (Catalog # 792-MG).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8066-GB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8066-GB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-4

IGFBP‑4 (insulin‑like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins that maintain IGF‑I and IGF‑II in the circulation and direct them to their target tissues (1, 2). Mouse IGFBP‑4 cDNA encodes 254 amino acids (aa) that include a 21 aa signal peptide and a 233 aa cysteine-rich mature protein that contains an N‑terminal IGFBP domain, a linker sequence, and a C‑terminal thyroglobulin domain. Mature mouse IGFBP‑4 shares 99% aa sequence identity with rat IGFBP‑4, and 90‑91% with human, canine, bovine, ovine and porcine IGFBP‑4. Both domains interact with IGFs, although the N-terminal domain is primary (2). The linker contains a cleavage site that is accessible by PAPP‑A (pregnancy-associated plasma protein A) when IGFs are bound (2, 3). IGFBP‑4 fragments detected in the plasma of pregnant women have lowered affinity for IGFs, thus up‑regulating IGF bioavailability for receptor binding (2). Unlike other family members, IGFBP‑4 does not interact with the extracellular matrix or translocate to the nucleus (2). Like other family members, it is expressed early in development and has both IGF‑dependent and ‑independent activities (2, 4). IGFBP‑4 expression in mesenchymal stem cells is up‑regulated by the cardiac transcription factor GATA‑4 (5). It then enhances cardiomyocyte differentiation by interacting with Frizzled and LRP molecules, inhibiting canonical Wnt signaling (6, 7). IGF can sequester IGFBP‑4 when present, inhibiting its ability to support cardiomyocyte differentiation (3, 4). IGFBP‑4 is also up‑regulated in osteoblasts in response to estrogen (8). Intact or proteolytically cleaved IGFBP‑4 may either promote or inhibit cancer cell growth, motility and invasion depending on cell type, tissue and stage of the cancer (9‑11). It is shown to inhibit IGF‑1‑ or FGF‑2‑induced angiogenesis, but not that induced by VEGF (10).

References

  1. Bethel, C.R. et al. (1994) Biochem. Mol. Biol. Int. 34:385.
  2. Forbes, B.E. et al. (2012) Front. Endocrinol. (Lausanne) 3:38.
  3. Gyrup, C. and C. Oxvig  (2007) Biochemistry 46:1972.
  4. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
  5. Li, H. et al. (2011) Cytotherapy 13:1057.
  6. Zhu, W. et al. (2008) Nature 454:345.
  7. Minato, A. et al. (2012) Biomaterials 33:515.
  8. Denger, S. et al. (2008) Mol. Endocrinol. 22:361.
  9. Ueno, K. et al. (2011) Int. J. Cancer 129:2360.
  10. Contois, L.W. et al. (2012) J. Biol. Chem. 287:1779.
  11. Moreno, M.J. et al. (2013) Neoplasia 15:554.

Long Name

Insulin-like Growth Factor Binding Protein 4

Alternate Names

IGFBP4

Entrez Gene IDs

3487 (Human); 16010 (Mouse)

Gene Symbol

IGFBP4

UniProt

P47879

Additional IGFBP-4 Products

Product Documents for Recombinant Mouse IGFBP-4 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8066-GB (with carrier)
Download SDS 8066-GB/CF (carrier free)

Product Specific Notices for Recombinant Mouse IGFBP-4 Protein

For research use only

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