Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9906-S4

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9906-S4-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q8R464

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IGSF4C/SynCAM4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IGSF4C/SynCAM4 protein
Mouse IGSF4C
(Gln25-Ala324)
Accession # Q8R464
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Glu26.

Predicted Molecular Mass

60 kDa

SDS-PAGE

81-93 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera, immobilized at 0.5-1 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein, CF

Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein Bioactivity

Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein Bioactivity

Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera (Catalog # 9906-S4) Induces Cortical Neurite Outgrowth. A) Untreated E16-18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-18 embryonic rat cortical neurons treated with 0.5 μg/mL of Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera.
Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein SDS-PAGE

Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IGSF4C was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 81-93 kDa and 160-190 kDa, respectively.

Formulation, Preparation and Storage

9906-S4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IGSF4C/SynCAM4

IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1-5). Each contain one V-type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca++-independent homophilic and heterophilic interactions. The 388  a mouse IGSF4C contains a 24 aa signal sequence, a 300 aa ECD that shares 99% amino acid (aa) identity with rat and human IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48-67 kDa, probably due to differences in glycosylation (2, 5, 6). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6-8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 7). IGSF4C is also proposed as a tumor suppressor that is down-regulated in many prostate cancers and gliomas (1, 5).

References

  1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
  2. Biederer, T. et al. (2006) Genomics 87:139.
  3. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  4. Thomas, L. A. et al. (2008) J. Comp. Neurol. 510:47.
  5. Williams, Y. N. et al. (2005) Oncogene 25:1446.
  6. Maurel, P. et al. (2007) J. Cell Biol. 178:861.
  7. Fogel, A. I. et al. (2007) J. Neurosci. 27:12516.
  8. Spiegel, I. et al. (2007) Nat. Neurosci. 10:861.

Long Name

Immunoglobulin Superfamily, Member 4B

Alternate Names

CADM4, Necl-4, SynCAM4, TSLC1-like 2, TSLL2

Entrez Gene IDs

199731 (Human); 260299 (Mouse)

Gene Symbol

CADM4

UniProt

Q8R464

Additional IGSF4C/SynCAM4 Products

Product Documents for Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.