Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11269-ML

Analyzed by SEC-MALS.
R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P43432.1 (p40) & Q9EQ14.1 (p19)

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-23 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IL-23 protein
Mouse IL-12p40
(Met22-Ser335)
Accession # P43432.1		 GSGSSRGGSGSGGSGGGGSKL Mouse IL-23p19
(Leu20-Ala196)
Accession # Q9EQ14.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22

Predicted Molecular Mass

57 kDa

SDS-PAGE

60-75 kDa, under reducing conditions.

Activity

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.050-0.500 ng/mL.

Scientific Data Images for Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF

Recombinant Mouse IL‑23 (HEK293-expressed) Protein SEC-MALS.

Recombinant Mouse IL-23 (Catalog # 11269-ML) has a molecular weight (MW) of 60.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Recombinant Mouse IL-23 (HEK293-expressed) Protein Bioactivity.

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.050-0.500 ng/mL.

Recombinant Mouse IL-23 (HEK293-expressed) Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse IL-23 (HEK293-expressed) Protein (Catalog # 11269-ML) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa.

Formulation, Preparation and Storage

11269-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human Tnbsp;cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Gene Symbol

IL23A

UniProt

P43432.1 (p40) & Q9EQ14.1 (p19)

Additional IL-23 Products

Product Documents for Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF

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