Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 530-MR

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q544A9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-4R alpha Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-4 R alpha protein
Mouse IL-4 R alpha
(Ile26 - Arg233)
Accession # Q544A9		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile26

Predicted Molecular Mass

51 kDa

SDS-PAGE

65-85 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 0.05-0.2 µg/mL in the presence of 3 ng/mL of recombinant mouse IL-4.

Formulation, Preparation and Storage

530-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4 R alpha), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 ‑ 5). Mature mouse IL-4 R alpha consists of a 208 amino acid (aa) extracellular domain (ECD) that contains a ligand binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 553 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6). Within the ECD, mouse IL-4 R alpha shares 51% and 76% aa sequence identity with human and rat IL-4 R alpha, respectively. Alternate splicing of mouse IL-4 R alpha generates a secreted isoform and an isoform that lacks the cytoplasmic region (6 - 8). Proteolytic cleavage can also release soluble IL-4 R alpha, and both these mechanisms produce a molecule which retains ligand binding properties and inhibits IL-4 bioactivity (6, 7, 9). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (10). It can associate with the common gamma chain ( gammac) to form the IL-4 responsive type I receptor in which gammac increases the affinity for IL-4 and enables signaling (11, 12). It can alternatively associate with IL-13 R alpha1 to form the type II receptor which is responsive to both IL-4 and IL-13 (13, 14). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gammac (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (15, 16).

References

  1. Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
  2. Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
  3. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
  4. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
  5. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
  6. Mosley, B. et al. (1989) Cell 59:335.
  7. Blum, H. et al. (1996) J. Immunol. 157:1846.
  8. Wrighton, N. et al. (1992) Growth Factors 6:103.
  9. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
  10. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
  11. Kondo, M. et al. (1993) Science 262:1874.
  12. Russell, S.M. et al. (1993) Science 262:1880.
  13. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
  14. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  15. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
  16. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse); 102133649 (Cynomolgus Monkey)

Gene Symbol

IL4R

UniProt

Q544A9

Additional IL-4R alpha Products

Product Documents for Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

For research use only

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