Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

R&D Systems, part of Bio-Techne | Catalog # 9038-SR

R&D Systems, part of Bio-Techne
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Carrier Free
9038-SR-025/CF
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With Carrier
9038-SR-025
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Key Product Details

Source

NS0

Accession #

P22272 (IL-6 R alpha) & P08505 (IL-6)

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-6/IL-6R alpha Complex Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-6/IL-6R alpha Complex protein
Mouse IL-6R alpha
(Leu20-Glu357)
Accession # P22272		 GGGSGGGSGGGS  Mouse IL-6
(Phe25-Thr211)
Accession # P08505
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20 (major), Leu28 (minor)

Predicted Molecular Mass

60 kDa

SDS-PAGE

70-87 kDa, reducing conditions

Activity

Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation.
The ED50 for this effect is 2-12 ng/mL.

Scientific Data Images for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Recombinant Mouse IL-6/IL-6R alpha Protein Chimera Bioactivity

Recombinant Mouse IL-6/IL-6R alpha Protein Chimera Bioactivity

Recombinant Mouse IL-6/IL-6R alpha complex (Catalog # 9038-SR) inhibits M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 2-12 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9038-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9038-SR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6/IL-6R alpha Complex

Interleukin-6 (IL-6) is a pleiotropic, alpha-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1-3). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 6). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (7). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (8). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (3, 9). Mature mouse IL-6 shares 39% and 85% amino acid (aa) sequence identity with human and rat IL-6, respectively (10-12). Within the extracellular domain, mouse IL-6 R alpha shares 51% and 89% aa sequence identity with human and rat IL-6 R alpha, respectively (13).

References

  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Hunter, C.A. and S.A. Jones (2015) Nat. Immunol. 16:448.
  4. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  5. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  6. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
  7. Murakami, M. et al. (1993) Science 260:1808.
  8. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  9. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  11. Simpson, R.J. et al. (1988) Eur. J. Biochem. 176:187.
  12. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
  13. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.

Long Name

Interleukin 6/Interleukin 6 Receptor alpha Complex

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat)

Gene Symbol

IL6R

UniProt

P22272 (IL-6 R alpha) & P08505 (IL-6)

Additional IL-6/IL-6R alpha Complex Products

Product Documents for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 9038-SR (with carrier)
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Product Specific Notices for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

For research use only

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