Recombinant Mouse IL-9 Protein

R&D Systems, part of Bio-Techne | Catalog # 409-ML

R&D Systems, part of Bio-Techne
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Carrier Free
409-ML-010/CF
409-ML-050/CF
Catalog #
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With Carrier
409-ML-010
409-ML-050
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P15247

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-9 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-9 protein
Gln19-Pro144

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained, sequencing might be blocked: Gln19 is predicted

Predicted Molecular Mass

14.2 kDa

SDS-PAGE

Multiple bands between 16-25 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.500-5.00 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 409-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 409-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gammac) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gammac to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively (14).

References

  1. Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
  2. Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
  3. Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
  4. Townsend, M.J. et al. (2000) Immunity 13:573.
  5. Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
  6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
  7. Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
  8. Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
  9. Tan, C. et al. (2010) J. Immunol. 185:6795.
  10. Jager, A. et al. (2009) J. Immunol. 183:7169.
  11. Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
  12. Lu, L.-F. et al. (2006) Nature 442:997.
  13. Stassen, M. et al. (2000) J. Immunol. 164:5549.
  14. Van Snick, J. et al. (1989) J. Exp. Med. 169:363.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

UniProt

P15247

Additional IL-9 Products

Product Documents for Recombinant Mouse IL-9 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 409-ML (with carrier)
Download SDS 409-ML/CF (carrier free)

Product Specific Notices for Recombinant Mouse IL-9 Protein

For research use only

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