Recombinant Mouse IL1RAPL1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10756-MR

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P59823.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL1RAPL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL1RAPL1 protein
Mouse IL1RAPL1
(Leu19-Thr357)
Accession # P59823.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu19

Predicted Molecular Mass

65 kDa

SDS-PAGE

78-88 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse IL1RAPL1 Fc Chimera (Catalog # 10756-MR) binds with an ED50 of 2.25-18.0 ng/mL.

Scientific Data Images for Recombinant Mouse IL1RAPL1 Fc Chimera Protein, CF

Recombinant Mouse IL1RAPL1 Fc Chimera Protein Binding Activity

When Recombinant Human PTPRD Fc Chimera (9995-PR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse IL1RAPL1 Fc Chimera (Catalog # 10756-MR) binds with an ED50 of 2.25-18.0 ng/mL.

Recombinant Mouse IL1RAPL1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse IL1RAPL1 Fc Chimera (Catalog # 10756-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-88 kDa and 156-176 kDa, respectively.

Formulation, Preparation and Storage

10756-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL1RAPL1

Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), also known as Oligophenin-4 (OPHN4) and three immunoglobulin domain containing IL-1 receptor-related 2 (TIGIRR-2) (1), is a member of the IL-1 receptor superfamily. IL1RAPL1 is a single pass type I membrane protein which contains an N-terminal signal peptide, three extracellular immunoglobulin-like domains, a transmembrane domain, an intracellular Toll/IL-1R domain, and a long C-terminal tail which interacts with multiple signaling molecules (2). High expression levels of IL1RAPL1 was found in post-natal hippocampus, and its expression is upregulated by neuronal activity (3). The extracellular domain of IL1RAPL1 can mediate synapse formation through trans-synaptic interaction with PTPRD (4, 5). In neurons, IL1RAPL1 interacts with PSD-95, a major scaffolding protein of excitatory synapses, and modulates its synaptic localization by regulating JNK activity and PSD-95 phosphorylation (3). Mutation or deletion of IL1RAPL1 gene is associated with non-syndromic intellectual disability and autism spectrum disorder (5). Mouse IL1RAPL1 shares 98% and 99% amino acid sequence identity with human and rat IL1RAPL1, respectively.

References

  1. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
  2. Bahi, N. et al. (2003) Hum. Mol. Gen. 12:1415.
  3. Pavlowsky, A. et al. (2010) Curr. Biol. 20:103.
  4. Yoshida, T. et al. (2011) J. Neurosci. 31:13485.
  5. Ramos-Brossier, M. et al. (2015) Hum Mol Genet. 24:1106.

Long Name

Interleukin 1 Receptor Accessory Protein-like 1

Alternate Names

IL-1R9, IL-1RAPL1, TIGIRR-2

Entrez Gene IDs

11141 (Human); 331461 (Mouse); 317553 (Rat)

Gene Symbol

IL1RAPL1

UniProt

P59823.1

Additional IL1RAPL1 Products

Product Documents for Recombinant Mouse IL1RAPL1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse IL1RAPL1 Fc Chimera Protein, CF

For research use only

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