Recombinant Mouse IMPAD1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7028-PD

R&D Systems, part of Bio-Techne
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7028-PD-050
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Key Product Details

Source

CHO

Accession #

Q80V26

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Inositol Monophosphatase 3/IMPAD1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Inositol Monophosphatase 3/IMPAD1 protein
Glu51-His356 with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result. Intact N-terminus verified by anti-poly-His Western.

Predicted Molecular Mass

34 kDa

SDS-PAGE

35-40 kDa, reducing conditions

Activity

Measured by its ability to dephosphorylate 3'-Phosphoadenosine 5'-phosphate.
The specific activity is >800 pmol/min/ug, as measured under the described conditions.

Formulation, Preparation and Storage

7028-PD
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Inositol Monophosphatase 3/IMPAD1

Sulfur metabolism is critical for multicellular organism development (1). Sulfur is activated in vivo to form the sulfur donor, 3'-phosphoadenosine-5'-phosphosulfate (PAPS) (2), and then transferred to acceptor molecules, such as glycosaminoglycans (3), steroids and xenobiotics (4). Following transfer, PAPS is converted to 3'-phosphoadenosine-5'-phosphate (PAP). PAP causes inhibition of many sulfotransferases and is therefore cytotoxic (5). IMPAD1 is a recently discovered PAP-specific phosphatase that removes the 3'-phosphate from PAP to form non-toxic AMP (6), which can subsequently be recycled by cells. The enzyme is localized in the Golgi apparatus where glycosaminoglycan synthesis occurs. Mouse IMPAD1 exhibits 91% sequence identity with the human homologue. R&D Systems has found that commercial preparations of PAP may contain trace lithium salts, which is a potent micromolar-level inhibitor of IMPAD1 (6).

References

  1. Stott, C.A. (2002) Endocr. Rev. 23:703.
  2. Robbins, P.W. and Lipmann, F. (1956) J. Am. Chem. Soc. 24:6409.
  3. Plaas, A. H. et al. (1998) J. Biol. Chem. 273:12642.
  4. Nowell, S. and Falany, C.N. (2006) Oncogene 25:1673.
  5. Gamage, N. et al. (2006) Toxicol. Sci. 90:5.
  6. Frederick, J.P. et al. (2008) Proc. Natl. Acad. Sci. USA 105:11605.

Alternate Names

IMP3, IMPA3, IMPase 3

Entrez Gene IDs

54928 (Human); 242291 (Mouse); 312952 (Rat)

Gene Symbol

BPNT2

UniProt

Q80V26

Additional Inositol Monophosphatase 3/IMPAD1 Products

Product Documents for Recombinant Mouse IMPAD1 Protein, CF

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Product Specific Notices for Recombinant Mouse IMPAD1 Protein, CF

For research use only

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