Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8615-A3
Key Product Details
Source
CHO
Structure / Form
Noncovalently-linked heterodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 4 beta 7/LPAM-1 protein
| Mouse Integrin alpha4 Tyr34-Gln970 (Arg591Leu) Accession # AAH68313 |
GS Linker | Acidic Tail | HHHHHH |
| Mouse Integrin beta7 Glu20-Arg724 Accession # P26011 |
GS Linker | Basic Tail | |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr34 (Integrin alpha4), Glu20 (Integrin beta7)
Predicted Molecular Mass
113 kDa (Integrin alpha4), 84 kDa (Integrin beta7)
SDS-PAGE
115 - 170 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.
The ED50 for this effect is 0.6-3.6 μg/mL.
The ED50 for this effect is 0.6-3.6 μg/mL.
Formulation, Preparation and Storage
8615-A3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Integrin alpha 4 beta 7/LPAM-1
alpha4 beta-propeller to form a binding domain. Metal ion binding sites termed MIDAS and LIMBS promote firm adhesion, and another site termed ADMIDAS is a negative regulatory site that promotes rolling (4-6). The mouse alpha4 ECD shares 94% and 85% amino acid sequence identity with the rat and human alpha4 ECD, respectively. The mouse beta7 ECD shares 94% and 87% amino acid sequence identity with the rat and human beta7 ECD, respectively. Integrin alpha4 beta7 binds the mucosal addressin
MAdCAM-1, as well as VCAM-1 and Fibronectin (7). Integrin alpha4 beta7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes (8, 9). Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid (10, 11). The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha4 beta7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) (12-14). Integrin alpha4 beta7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) (15).
References
- Luo, B.H. et al. (2007) Annu. Rev. Immunol. 25:619.
- Takada, Y. et al. (1989) EMBO J. 8:1361.
- Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.
- Chen, J. et al. (2004) J. Biol. Chem. 279:55556.
- Park, E.J. et al. (2007) J. Clin. Invest. 117:2526.
- Yu, Y. et al. (2012) J. Cell Biol. 196:131.
- Yang, Y. et al. (1998) Eur. J. Immunol. 28:995.
- Wagner, N. et al. (1996) Nature 382:366.
- Johansson-Lindbom, B. and W.W. Agace (2007) Immunol. Rev. 215:226.
- Hammerschmidt, S.I. et al. (2008) J. Exp. Med. 205:2483.
- Edele, F. et al. (2008) J. Immunol. 181:3745.
- Arthos, J. et al. (2008) Nat. Immunol. 9:301.
- Cicala, C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20877.
- Monteiro, P. et al. (2011) J. Immunol. 186:4618.
- Chen, Y.B. et al. (2013) Bone Marrow Transplant. 48:598.
Alternate Names
CD49d/beta7, LPAM-1, LPAM1
Gene Symbol
ITGA4
Additional Integrin alpha 4 beta 7/LPAM-1 Products
Product Documents for Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF
Product Specific Notices for Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF
For research use only
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