Recombinant Mouse Kallikrein 5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7236-SE

R&D Systems, part of Bio-Techne
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7236-SE-010
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Key Product Details

Source

NS0

Accession #

NP_081082

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Kallikrein 5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Kallikrein 5 protein
Val23-Asn293, with a C-terminal 10‑His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23

Predicted Molecular Mass

31 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is >1,350 pmol/min/μg, as measured under the described conditions. www.RnDSystems.com.

Formulation, Preparation and Storage

7236-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 5

Tissue Kallikrein 5 (KLK5) is a secreted serine protease with trypsin‑like substrate specificity. KLK5 is highly expressed in the skin, and is also known as stratum corneum tryptic enzyme due to its high expression level in this tissue. KLK5 is thought to play an important role in the process of desquamation (1). KLK5 is a potential biomarker for ovarian and breast cancers (2). It may also be a physiological activator of KLK7 in the stratum corneum (3). Recombinant mouse KLK5 is purified as the proform, and can undergo autoactivation under the appropriate conditions. KLK5 enzymatic activity can be inhibited by the following recombinant human serpins: Serpin‑A1, ‑A5, ‑F2, and ‑G1 (Catalog # http://www.rndsystems.com/product_results.aspx?k=1268-PI">1268-PI, http://www.rndsystems.com/product_results.aspx?k=1266-PI">1266-PI, http://www.rndsystems.com/product_results.aspx?k=1470-PI">1470-PI, and http://www.rndsystems.com/product_results.aspx?k=2488-PI">2488-PI.

References

  1. Brattsand M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
  2. Yousef G.M. et al. (2003) Cancer Res. 63:3958.
  3. Kim H. et al. (2001) Br. J. Cancer 84:543.

Alternate Names

KLK-L2, KLK5, SCTE

Entrez Gene IDs

25818 (Human); 68668 (Mouse); 690603 (Rat)

Gene Symbol

KLK5

UniProt

NP_081082

Additional Kallikrein 5 Products

Product Documents for Recombinant Mouse Kallikrein 5 Protein, CF

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Product Specific Notices for Recombinant Mouse Kallikrein 5 Protein, CF

For research use only

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