Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9095-T4

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9095-T4-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q64281

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRB4/CD85k/ILT3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LILRB4/CD85k/ILT3 protein
Gly24-Lys238, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24 & Ser37 (minor)

Predicted Molecular Mass

25 kDa

SDS-PAGE

34-42 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse LILRB4/CD85k/ILT3 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 20-120 ng/mL.

Scientific Data Images for Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

Recombinant Mouse LILRB4/CD85k/ILT3 Protein Bioactivity

Recombinant Mouse LILRB4/CD85k/ILT3 Protein Bioactivity

When Recombinant Mouse LILRB4/CD85k/ ILT3 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of 20-120 ng/mL.

Formulation, Preparation and Storage

9095-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB4/CD85k/ILT3

LILRB4, also known as ILT3, CD85k, and LIR-5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment (2). LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (3, 6, 7). Ligation of LILRB4 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (3, 5, 8, 9). Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (5, 8, 10). In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (6, 7, 9, 11, 12).

References

  1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
  2. Castells, M.C. et al. (1994) J. Biol. Chem. 269:8393.
  3. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
  4. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
  5. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
  6. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
  7. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
  8. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
  9. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  10. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
  11. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  12. Brenk, M. et al. (2009) J. Immunol. 183:145.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Alternate Names

CD85k, HM18, ILT3, LIR5

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Gene Symbol

LILRB4

UniProt

Q64281

Additional LILRB4/CD85k/ILT3 Products

Product Documents for Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.