Recombinant Mouse LRRN4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10411-LR

R&D Systems, part of Bio-Techne
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10411-LR-050
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Key Product Details

Source

NS0

Accession #

P59383.2

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRN4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LRRN4 protein
Gln22-Ser676, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23

Predicted Molecular Mass

72 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse LRRN4 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Mouse LRRN4 His-tag Protein, CF

Recombinant Mouse LRRN4 His-tag Protein SDS-PAGE

Recombinant Mouse LRRN4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse LRRN4 His-tag (Catalog # 10411-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa.

Formulation, Preparation and Storage

10411-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRN4

LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature mouse LRRN4 is composed of a 657 amino acid (aa) extracellular domain (ECD) that includes ten LRRs, one LRRCT and a fibronectin type-III like domain, a 21 aa transmembrane segment, and a 36 aa cytoplasmic domain. Within the ECD, mouse LRRN4 shares 66% and 84% aa sequence identity with human and rat LRRN4, respectively. LRRN4-deficient mice show defects in the memory retention, suggesting this protein may play an important role in hippocampus-dependent long-lasting memory (1). In addition, LRRN4 is found in about 8% dorsal root ganglion (DRG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a maker for detection of mesothelioma antigens (4).

References

  1. Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
  2. Bando, T. et al. (2012) Neurosci. Lett. 531:24.
  3. Bando, T. et al. (2013) Neurosci. Lett. 548:73.
  4. Kanamori-Katayama, M. (2011) PloS. One. 6(10):e24391.

Long Name

Leucine-rich Repeat Neuronal 4

Alternate Names

C20orf75, dJ1056H1.1, NLRR4

Entrez Gene IDs

164312 (Human); 320974 (Mouse); 311443 (Rat)

Gene Symbol

LRRN4

UniProt

P59383.2

Additional LRRN4 Products

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Product Specific Notices for Recombinant Mouse LRRN4 His-tag Protein, CF

For research use only

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