Recombinant Mouse Lysyl Oxidase Homolog 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9259-AO

R&D Systems, part of Bio-Techne
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9259-AO-020
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Key Product Details

Source

NS0

Accession #

P58022

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Lysyl Oxidase Homolog 2/LOXL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Lysyl Oxidase Homolog 2/LOXL2 protein
Gln26-Gln776
with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln26 inferred from enzymatic pyroglutamate treatment revealing Tyr27

Predicted Molecular Mass

85 kDa

SDS-PAGE

93-111 kDa, reducing conditions

Activity

Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine.
The specific activity is >4.5 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Mouse Lysyl Oxidase Homolog 2 Protein, CF

Recombinant Mouse Lysyl Oxidase Homolog 2 Protein SDS-PAGE

Recombinant Mouse Lysyl Oxidase Homolog 2 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse Lysyl Oxidase Homolog 2 (Catalog # 9259-AO) and 1 μg/lane of competitor Lysyl Oxidase Homolog 2 was resolved with 4-20% SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining. The competitor product is predominantly truncated/degraded material.

Formulation, Preparation and Storage

9259-AO
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Lysyl Oxidase Homolog 2/LOXL2

Lysyl Oxidase Homolog 2 (lysyl oxidase-like protein 2, LOXL2) is a member of lysyl oxidase-like (LOXL) gene family which includes LOXL1 through LOXL4. These enzymes are secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes (1). The N-terminal region of LOXL2 contains four scavenger receptor cysteine-rich (SRCR) domains, and the C-terminal region is a catalytic domain similar to other lysyl oxidases (1). The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone co-factor (2). It has been shown that LOXL2 promotes cell migration and tumor cell invasiveness (3, 4). Elevated expression of LOXL2 is also associated with cancer progression in various tumors and carcinoma cell lines, which makes it a potential marker for prognosis of cancer (5). LOXL2 is expressed in many tissues, with elevated levels in reproductive tissues such as placenta, uterus, and prostate (6). Activity is routinely determined using benzylamine as a substrate, although significantly higher activity is detected when activity is quantified using lysine as the substrate.

References

  1. Csiszar, H. (2001) Prog. Nucleic Acid Res. Mol. Biol. 70:1.
  2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
  3. Akiri, G. et al. (2003) Cancer Res. 63:1657.
  4. Hollosi, P. et al. (2009) Int. J. Cancer. 125:318.
  5. Peinado, H. et al. (2008) Cancer Res. 68:4541.
  6. Jourdan-Le Saux C. et al. (1999) J. Biol. Chem. 274:12939.

Alternate Names

LOL2, LOXL2

Entrez Gene IDs

4017 (Human); 94352 (Mouse); 102138804 (Cynomolgus Monkey)

Gene Symbol

LOXL2

UniProt

P58022

Additional Lysyl Oxidase Homolog 2/LOXL2 Products

Product Documents for Recombinant Mouse Lysyl Oxidase Homolog 2 Protein, CF

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Product Specific Notices for Recombinant Mouse Lysyl Oxidase Homolog 2 Protein, CF

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