Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 993-MC
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse MAdCAM-1 protein
| Mouse MAdCAM-1 (Gln22 - Thr365) Accession # NP_038619 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln22 predicted
Predicted Molecular Mass
63 kDa (monomer)
SDS-PAGE
85-95 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells.
When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
993-MC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MAdCAM-1
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha4 beta7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha4 beta7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha4 beta7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha4 beta7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.
References
- Briskin, M.J. et al. (1993) Nature 363:461.
- Yang, X.D. et al. (1997) Diabetes 46:1542.
- Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
- Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
- Berg, E.L. et al. (1993) Nature 366:695.
- Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.
Long Name
Mucosal Addressin Cell Adhesion Molecule 1
Alternate Names
MAdCAM1
Gene Symbol
MADCAM1
UniProt
Additional MAdCAM-1 Products
Product Documents for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF
For research use only
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