Recombinant Mouse Mast Cell Protease-11/Prss34 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2857-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Mast Cell Protease-11/Prss34 protein
Met20-Ser318, with a C-terminal 10-His tag
Met20-Ser318, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met20
Predicted Molecular Mass
34 kDa
SDS-PAGE
48 kDa doublet, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2857-SE
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Mast Cell Protease-11/Prss34
Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The mouse MCP-11 (residues 20 to 318) was expressed in the NS0 cells with a foreign signal peptide. After being treated with thermolysin, the purified enzyme is active against a peptide substrate described in the Activity Assay Protocol. Apparently, the human gene corresponding to Prss34 encodes a protein that is not enzymatically active due to a mutation that leads to a premature translation termination codon.
References
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
Alternate Names
Mast Cell Protease11, MCP-11, MCPT11, Prss34
Entrez Gene IDs
328780 (Mouse)
Gene Symbol
PRSS34
UniProt
Additional Mast Cell Protease-11/Prss34 Products
Product Documents for Recombinant Mouse Mast Cell Protease-11/Prss34 Protein, CF
Product Specific Notices for Recombinant Mouse Mast Cell Protease-11/Prss34 Protein, CF
For research use only
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