Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF
R&D Systems, part of Bio-Techne | Catalog # 4735-SE
Key Product Details
Product Specifications
Source
E. coli-derived mouse Matriptase/ST14 protein
Gly596-Val855, with an N-terminal Met and 6-His tag
The protein was auto-activated and further purified.
Gly596-Val855, with an N-terminal Met and 6-His tag
The protein was auto-activated and further purified.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met & Val615
Predicted Molecular Mass
3 kDa & 26 kDa
SDS-PAGE
27 kDa (major) and minor auto-activation fragments, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 4735-SE in the following applications:
Formulation, Preparation and Storage
4735-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Matriptase/ST14
Matriptase, a mouse type II membrane serine protease encoded by the ST14 (suppression of tumorigenicity 14) gene, is also known as epithin, and membrane-type serine protease 1/MT-SP1 (1-2). Its human ortholog MT-SP1/Matriptase (Catalog # http://www.rndsystems.com/product_results.aspx?k=3946-SE">3946-SE), which shares 81% amino acid identity with epithin, has been thought to play an important role in tumor biology and is a potential target for anti-cancer therapy (3). Matriptase has a multidomain structure containing a putative N-terminal transmembrane region, two CUB domains, four LDLRA repeats, and a C-terminal serine protease domain (1). The protease domain of epithin starts with Val615. R&D Systems recombinant mouse Matriptase is an active protease and consists of the catalytic domain (Val615 to Val855) and a short peptide (Gly596 to Arg614).
References
- Cho, E. et al. (2001) J. Biol. Chem. 276:44581.
- List, K. et al. (2006) Mol. Med. 12:1.
- Uhland, K. (2006) Cell Mol. Life Sci. 63:2968.
Alternate Names
Epithin, MTSP1, PRSS14, SNC19, ST14, TADG-15, TMPRSS14
Gene Symbol
ST14
UniProt
Additional Matriptase/ST14 Products
Product Documents for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF
Product Specific Notices for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF
For research use only
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