Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8604-MS

C-Terminal
R&D Systems, part of Bio-Techne
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8604-MS-050
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Key Product Details

Source

CHO

Accession #

Q61468

Conjugate

Unconjugated

Applications

Bioactivity

View all Mesothelin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Mesothelin protein
Asp298-Ser600, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp298

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Mesothelin C-Terminal (aa 298-600) His-tag (Catalog 8604-MS) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 (Catalog # 5609-MU) that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.

Reviewed Applications

Read 3 reviews rated 5 using 8604-MS in the following applications:

Formulation, Preparation and Storage

8604-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 6). It is up-regulated in mesotheliomas and a range of carcinomas and adenomas (7-10). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7 (9, 11, 12). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages (10, 14-16).

References

  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
  3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
  5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
  6. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
  7. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
  8. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
  9. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
  10. Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
  11. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
  12. Chang, M.-C. et al. (2012) Biochem. J. 442:293.
  13. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  14. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
  15. Robinson, B.W.S. et al. (2003) Lancet 362:1612.
  16. Dangaj, D. et al. (2011) PLoS ONE 6:e28386.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

Q61468

Additional Mesothelin Products

Product Documents for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

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