Recombinant Mouse MMP-8 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2904-MP
Key Product Details
Source
CHO
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse MMP-8 protein
Phe21-Ser465, with a C-terminal 6-His tag
Phe21-Ser465, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe21 & Leu27
Predicted Molecular Mass
52 kDa and 51 kDa
SDS-PAGE
60-65 kDa doublet, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 3 reviews rated 5 using 2904-MP in the following applications:
Formulation, Preparation and Storage
2904-MP
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2 and Brij-35. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MMP-8
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade many components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta-casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain.
Long Name
Matrix Metalloproteinase 8
Alternate Names
Collagenase 2, MMP8
Gene Symbol
MMP8
UniProt
Additional MMP-8 Products
Product Documents for Recombinant Mouse MMP-8 Protein, CF
Product Specific Notices for Recombinant Mouse MMP-8 Protein, CF
For research use only
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