Recombinant Mouse MOG Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8536-MO
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse MOG protein
Gly29-Gly153, with a C-terminal 10-His tag
Gly29-Gly153, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly29
Predicted Molecular Mass
16 kDa
SDS-PAGE
19-29 kDa, reducing conditions
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse MOG, immobilized at 1 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Recombinant Mouse MOG, immobilized at 1 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Formulation, Preparation and Storage
8536-MO
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MOG
Myelin oligodendrocyte glycoprotein (MOG) is 28 kDa single-pass transmembrane glycoprotein that is a member of the Ig superfamily (1-4). Mouse MOG is synthesized with a 28 amino acid (aa) signal sequence, a 128 aa extracellular domain (ECD) containing an Ig-like domain, a 21 aa transmembrane domain, and a 69 aa cytosolic fragment featuring a hydrophobic domain that associates with the cytoplasmic face of the plasma membrane. The ECD of mature mouse MOG shares 90% and 95% aa sequence identity with the ECD of human and rat MOG, respectively. Dimerization of MOG occurs via the extracellular Ig-like domain (5-8). MOG is expressed exclusively by oligodendrocytes in the central nervous system (CNS) and is localized to the outer layer of the myelin sheath as well as in the oligodendrocyte plasma membrane (9). MOG expression in the brain can be used as a temporal biomarker for myelin development. MOG is an important antigenic target for autoimmune diseases that mediate demyelination in the CNS (10). In vivo administration of exogenous MOG protein or peptide induces experimental autoimmune encephalomyelitis (EAE) in multiple animal species (11, 12). EAE is used as an animal model for multiple sclerosis and related CNS demyelinating diseases. MOG is thought function as an adhesion molecule as well as a mediator of immune activation in the CNS (2, 9, 13).
References
- Johns, T.G. and C.C. Bernard (1999) J. Neurochem. 72:1.
- Pham-Dinh, D. et al. (1993) Proc. Natl. Acad. Sci. U S A 90:7990.
- Hilton, A.A. et al. (1995) J. Neurochem. 65:309.
- Slavin, A.J. et al. (1997) Dev. Neurosci. 19:69.
- Abo, S. et al. (1993) Biochem. Mol. Biol. Int. 30:945.
- Amiguet, P. et al. (1992) J. Neurochem. 58:1676.
- Bettadapura, J. et al. (1998) J. Neurochem. 70:1593.
- Clements, C.S. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:11059.
- Reindl, M. et al. (2013) Nat. Rev. Neurol. 9:455.
- von Budingen, H.C. et al. (2004) Eur. J. Immunol. 34:2072.
- Rangachari, M. and V.K. Kuchroo (2013) J. Autoimmun. 45:31.
- Tompkins, S.M. et al. (2002) J. Immunol. 168:4173.
- Garcia-Vallejo, J.J. et al. (2014) J. Exp. Med. 211:1465.
Long Name
Myelin Oligodendrocyte Glycoprotein
Alternate Names
MGC26137, MOG Ig-AluB, MOGIG2, myelin oligodendrocyte glycoprotein, myelin-oligodendrocyte glycoprotein
Gene Symbol
MOG
UniProt
Additional MOG Products
Product Documents for Recombinant Mouse MOG Protein, CF
Product Specific Notices for Recombinant Mouse MOG Protein, CF
For research use only
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