Recombinant Mouse Neogenin Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1079-NE

R&D Systems, part of Bio-Techne
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1079-NE-050
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Key Product Details

Source

NS0

Accession #

NP_032710

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all Neogenin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Neogenin protein
Mouse Neogenin
(Ala42-Ile1033)
(Asp442-Leu461 del)
Accession # NP_032710		 IEGRID Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala42

Predicted Molecular Mass

133 kDa (monomer)

SDS-PAGE

155-175 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse Neogenin Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Chicken Netrin‑1 (Catalog # 128-N1) with a linear range of 6-400 ng/mL.

Formulation, Preparation and Storage

1079-NE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neogenin

Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2-type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the mouse protein shares 91% and 94% amino acid sequence identity with the human and rat orthologs, respectively (1). Five different isoforms are produced from alternative splicing of mouse Neo1. Neogenin is widely expressed in both neuronal and non-neuronal tissues of the developing mouse embryo, and in most tissues in adult mice (2). It is a multifunctional cell-surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates
Netrin-1-dependent attraction and RGM-A-dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorgenesis and cancer cell invasiveness in brain and gastric cancers (12-14).

References

  1. Meyerhardt, J.A. et al. (1997) Oncogene 14:1129.
  2. Keeling, S.L. et al. (1997) Oncogene 15:691.
  3. Hagihara, M. et al. (2011) J. Biol. Chem. 286:5157.
  4. Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700.
  5. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  6. van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828.
  7. De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483.
  8. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
  9. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  10. de Castro, F. (2003) News Physiol. Sci. 18:130.
  11. O’Leary, C.J. et al. (2015) Stem Cells 33:503.
  12. Milla, L.A. et al. (2014) Int. J. Cancer 134:21.
  13. Akino, T. et al. (2014) Cancer Res. 74:3716.
  14. Kim, S.J. et al. (2014) Oncotarget 5:3386.

Alternate Names

NEO, NEO1, NGN

Entrez Gene IDs

4756 (Human); 18007 (Mouse)

Gene Symbol

NEO1

UniProt

NP_032710

Additional Neogenin Products

Product Documents for Recombinant Mouse Neogenin Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Neogenin Fc Chimera Protein, CF

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