Recombinant Mouse Neuregulin-1/NRG1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9875-NR

R&D Systems, part of Bio-Techne
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9875-NR-050
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Key Product Details

Source

NS0

Accession #

XP_006509143

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuregulin-1/NRG1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Neuregulin-1/NRG1 protein
Ser20-Lys246, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

26 kDa

SDS-PAGE

42-49 kDa, reducing conditions

Activity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 0.9-5.4 ng/mL.

Scientific Data Images for Recombinant Mouse Neuregulin-1/NRG1 Protein, CF

Recombinant Mouse Neuregulin-1/NRG1 Protein Bioactivity

Recombinant Mouse Neuregulin-1/NRG1 Protein Bioactivity

Recombinant Mouse Neuregulin-1/NRG1 (Catalog # 9875-NR) stimulates MCF-7 human breast cancer cell proliferation. The ED50 for this effect is 0.9-5.4 ng/mL.
Recombinant Mouse Neuregulin-1/NRG1 Protein SDS-PAGE

Recombinant Mouse Neuregulin-1/NRG1 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Neuregulin-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-49 kDa.

Formulation, Preparation and Storage

9875-NR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Neuregulin-1/NRG1

Neuregulin-1 (NRG1) belongs to a family of structurally related glycoproteins encoded by four distinct but related genes, Nrg1, Nrg2, Nrg3, and Nrg4. In humans, Nrg1 encodes multiple soluble or transmembrane proteins through alternative splicing or the use of alternative promoters with each NRG1 isoform exhibiting distinct expression patterns and functions (1). Type I isoforms include Neu Differentiation Factor, Heregulin, and ARIA, and they consist of an N-terminal domain, an Ig-like domain, a linker with a Ser/Thr rich region, an EGF-like domain, a transmembrane segment, and a cytoplasmic domain. Type II isoforms have a larger N-terminal domain and lack the Ser/Thr rich linker, while Type III isoforms lack the Ig-like domain but contain a cysteine rich domain (CRD) and a second transmembrane segment (1-6). The mature mouse NRG1 isoform expressed corresponds to Heregulin-beta 1 with an extracellular domain of 229 amino acids (aa) that shares 92% and 79% sequence identity with human and rat NRG1, respectively. NRG1 regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination (1-3). All NRG1 isoforms share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors to induce signaling (1-4). Heregulin-beta 1 has been directly linked to diverse functions including cardiovascular development and protection from ischemic injury (7) to a role in the growth and maintenance of human embryonic stem cells (8). Multiple polymorphisms and aberrant expression of NRG1 isoforms are associated with the development of schizophrenia and several cancers (2, 9, 10). Activation of Heregulin-beta 1 signaling has been shown to promote invasiveness and metastasis of breast cancer cells (11, 12).

References

  1. Meyer, D. et al. (1997) Development 124:3575.
  2. Mei, L. and W.-C. Xiong (2008) Nat. Rev. Neurosci. 9:437.
  3. Talmage, D.A. (2008) Novartis Found. Symp. 289:74.
  4. Holmes, W.E. et al. (1992) Science 256:1205.
  5. Marchionni, M.A. et al. (1993) Nature 362:312.
  6. Ho, W.-H. et al. (1995) J. Biol. Chem. 270:14523.
  7. Odiete et al. (2012) Circ Res. 111(10):1376.
  8. Wang, L. et al. (2007) Blood. Dec 1; 110(12):4111.
  9. Harrison PJ and Law AJ. (2006) Biol Psychiatry 60(2):132.
  10. Breleux, M. (2007) Cell. Mol. Life Sci. 64:2358.
  11. Atlas, E. et al. (2003) Mol Cancer Res. 2003(3):165.
  12. Hutcheson IR et al. (2007) Breast Cancer Res. 9(4):R50.

Alternate Names

Heregulin-1, HGL, HRG1-alpha, HRG1-beta 1, Neuregulin1, NRG1

Entrez Gene IDs

3084 (Human); 211323 (Mouse); 112400 (Rat)

Gene Symbol

NRG1

UniProt

XP_006509143

Additional Neuregulin-1/NRG1 Products

Product Documents for Recombinant Mouse Neuregulin-1/NRG1 Protein, CF

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Product Specific Notices for Recombinant Mouse Neuregulin-1/NRG1 Protein, CF

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