Recombinant Mouse Neurocan Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5800-NC

R&D Systems, part of Bio-Techne
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5800-NC-050
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Key Product Details

Source

CHO

Accession #

P55066

Conjugate

Unconjugated

Applications

Bioactivity

View all Neurocan Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Neurocan protein
Asp23-Asp637, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23

Predicted Molecular Mass

67.1 kDa

SDS-PAGE

120-200 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.

When 5 x 104 cells/well are added to mouse Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is
0.1-0.4 μg/mL.

Formulation, Preparation and Storage

5800-NC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurocan

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37 - 157), two Link domains (aa 159 - 254 and 258 - 356), two EGF-like domains (aa 960 - 996 and 998 - 1034), one C-type lectin-like domain (aa 1036 - 1165), one Sushi domain (aa 1165 - 1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2 - 3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

References

  1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
  2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
  4. Quaglia, X. et al. (2008) Brain 131:240.

Alternate Names

CSPG3, NCAN, NEUR

Entrez Gene IDs

1463 (Human); 13004 (Mouse); 58982 (Rat)

Gene Symbol

NCAN

UniProt

P55066

Additional Neurocan Products

Product Documents for Recombinant Mouse Neurocan Protein, CF

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Product Specific Notices for Recombinant Mouse Neurocan Protein, CF

For research use only

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