Recombinant Mouse Notum Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9150-NO

R&D Systems, part of Bio-Techne
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9150-NO-050
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Key Product Details

Source

NS0

Accession #

Q8R116

Conjugate

Unconjugated

Applications

Bioactivity

View all Notum Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Notum protein
Ser88-Thr458 (Cys337Ser), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser88, Ser239

Predicted Molecular Mass

43 kDa

SDS-PAGE

39-46 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
The ED50 for this effect is 0.2-1.2  μg/mL in the presence of 0.1 μg/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Images for Recombinant Mouse Notum Protein, CF

Recombinant Mouse Notum Protein Bioactivity

Recombinant Mouse Notum Protein Bioactivity

Recombinant Mouse Wnt-3a shows a dose responsive increase in Wnt reporter activity in HEK293 cells (green line). Recombinant Mouse Notum (Catalog # 9150-NO) inhibits a constant dose of 0.1 µg/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN) . The ED50 is 0.2-1.2 µg/mL (orange line).

Formulation, Preparation and Storage

9150-NO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notum

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively.

References

  1. Zhang, X. et al. (2015) Dev. Cell 32:719.
  2. Kakugawa, S. et al. (2015) Nature 519:187.
  3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
  4. Traister, A. et al. (2008) Biochem. J. 410:503.
  5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
  6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
  7. De Robertis, M. et al. (2015) Oncotarget 6:41237.

Alternate Names

EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog

Entrez Gene IDs

147111 (Human); 77583 (Mouse); 303743 (Rat)

Gene Symbol

NOTUM

UniProt

Q8R116

Additional Notum Products

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