Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10000-TA

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

B6A8R8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all OLT-2/TARM1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse OLT-2/TARM1 protein
Mouse TARM-1
(Gln17-Ile257)
Accession # B6A8R8
 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln17 predicted.

Predicted Molecular Mass

53 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-10 μg/mL.

Scientific Data Images for Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF

Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein Bioactivity

Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein Bioactivity

Recombinant Mouse OLT-2/TARM1 Fc Chimera (10000-TA) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.
Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein SDS-PAGE

Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse OLT-2/TARM1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-80 kDa and 140-160 kDa, respectively.

Formulation, Preparation and Storage

10000-TA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: OLT-2/TARM1

TARM-1 (T cell interacting, activating receptor on myeloid cells-1), also name OLT-2 (OSCAR-like Transcript 2), is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse TARM-1 contains a 242 amino acid (aa) long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of mouse TARM-1 shares 81.6% and 46.5% aa identity with rat and human TARM-1 respectively. TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12. TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. TARM1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. TARM1 Fc fusion protein inhibits anti-CD3 induced CD4+ cell activation, suggesting that TARM1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

References

  1. Radjabova, V. et al. (2015) J Immunol. 195:3149.

Long Name

T-cell-interacting Activating Receptor on Myeloid Cells 1

Alternate Names

OLT2, TARM1

Entrez Gene IDs

441864 (Human); 245126 (Mouse); 499065 (Rat)

Gene Symbol

TARM1

UniProt

B6A8R8

Additional OLT-2/TARM1 Products

Product Documents for Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF

For research use only

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