Recombinant Mouse OSMR beta Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 662-OR

R&D Systems, part of Bio-Techne
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662-OR-100
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Key Product Details

Source

NS0

Accession #

O70458

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all OSMR beta Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse OSMR beta protein
Mouse OSM R beta
(Glu24-Leu738)
Accession # O70458		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24

Predicted Molecular Mass

109 kDa (monomer)

SDS-PAGE

120-150 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse OSM R beta Fc Chimera is immobilized at 0.5 µg/mL and Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG) at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) that produces 50% of the optimal binding response is approximately 0.2‑1 ng/mL.

Formulation, Preparation and Storage

662-OR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OSMR beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta) and gp130, the type II complex made up of OSM receptor beta (OSM R beta) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

References

  1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
  2. Tanaka, M. et al. (1999) Blood 93:804.

Long Name

Oncostatin M Receptor beta

Alternate Names

OSM R beta, OSMR, OSMRB

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Gene Symbol

OSMR

UniProt

O70458

Additional OSMR beta Products

Product Documents for Recombinant Mouse OSMR beta Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse OSMR beta Fc Chimera Protein, CF

For research use only

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