Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI1019

R&D Systems, part of Bio-Techne
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AVI1019-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q9EP73.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all PD-L1/B7-H1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse PD-L1/B7-H1 protein
Mouse B7-H1
(Phe19-Thr238)
Accession # Q9EP73.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

53 kDa

SDS-PAGE

67-85 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PD-1 Fc Chimera Protein (Catalog # 1021-PD) is immobilized at 2.50 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI1019) binds with an ED50 of 0.0600-0.600 μg/mL.

Scientific Data Images for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-1 Fc Chimera Protein (1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED50 of 0.0600-0.600 μg/mL.

Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-85 kDa and 130-170 kDa, respectively.

Formulation, Preparation and Storage

AVI1019
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7,10,13). Our Avi-tag Biotinylated mouse B7-H1 Fc Chimeria features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Tamura, H. et al. (2001) Blood 97:1809.
  3. Chen, L. et al. (2007) J. Immunol. 178:6634.
  4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  5. Tsushima, F. et al. (2007) Blood 110:180.
  6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  9. Dong, H. et al. (2002) Nat. Med. 8:793.
  10. Azuma, T. et al. (2008) Blood 111:3635.
  11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  12. Park, J.-J. et al. (2010) Blood 116:1291.
  13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  14. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9EP73.1

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein, CF

For research use only

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