Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6339-SE
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Prolyl Oligopeptidase/PREP protein
Leu2-Gln710, with an N-terminal Met and 6-His tag
Accession # Q9QUR6
Leu2-Gln710, with an N-terminal Met and 6-His tag
Accession # Q9QUR6
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
82 kDa
SDS-PAGE
68-73 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).
The specific activity is >4,500 pmol/min/μg, as measured under the described conditions.
The specific activity is >4,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6339-SE
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Prolyl Oligopeptidase/PREP
Prolyl Oligopeptidase, also known as prolyl endopeptidase and post-proline cleaving enzyme, is a serine peptidase displaying specificity for the cleavage of Pro-Xaa bonds of oligopeptide substrates (1, 2). The peptidase is known to hydrolyze a variety of biologically active peptides such as bradykinin, substance P, neurotensin, and vasopressin (3). Because of its action on neuropeptides, Prolyl Oligopeptidase is considered to be involved in processes such as learning, memory, and depression (4).
References
- Szeltner, Z and L. Polgár (2008) Current Protein Pept. Sci. 9:96.
- Yoshimoto, T. et al. (1977) Biochemistry. 16:2942.
- Wilk, S. (1983) Life Sci. 33:2149.
- Männisto, P.T. et al. (2007) Drug News Perspect. 20:293.
Alternate Names
PE, PREP, rPop
Gene Symbol
PREP
UniProt
Additional Prolyl Oligopeptidase/PREP Products
Product Documents for Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF
Product Specific Notices for Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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