Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9258-SE

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q80W65

Structure / Form

Pro domain & mature form

Conjugate

Unconjugated

Applications

Bioactivity

View all Proprotein Convertase 9/PCSK9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Proprotein Convertase 9/PCSK9 protein
Ser156-Gln694 with a C-terminal 10-His tag; Gln35-Gln155

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser156 (mature form); No results obtained for pro domain, Gln35 inferred from enzymatic pyroglutamate treatment revealing Asp36

Predicted Molecular Mass

14 kDa (pro domain) and 59 kDa (mature form)

SDS-PAGE

18 kDa (pro domain) and 57-67 kDa (mature form), reducing conditions 

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse LDLR (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog #
9258-SE) binds with an ED50 = 25-150 ng/mL.

Scientific Data Images for Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein Bioactivity

Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein Bioactivity

Recombinant Mouse Proprotein Convertase 9/ PCSK9 Binds to Recombinant Mouse LDL R in an ELISA Binding Assay. When Recombinant Mouse LDL R Recombinant Mouse LDL R (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog # 9258-SE) binds with an ED50 = 25-150 ng/mL.

Formulation, Preparation and Storage

9258-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage


Background: Proprotein Convertase 9/PCSK9

PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain. Mouse PCSK9 shares 78% and 93% amino acid identity with human and rat PCSK9, respectively. PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (3-8). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (9-11). The ability of PCSK9 to regulate LDL R expression is inhibited by its binding to LDL particles or Annexin A2 or by additional proteolytic cleavage (12-17).

References

  1. Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
  2. Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865.
  3. Lagace, T.A. et al. (2006) J. Clin. Invest. 116:2995.
  4. Grefhorst, A. et al. (2008) J. Lipid Res. 49:1303.
  5. Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
  6. DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
  7. Canuel, M. et al. (2013) PLoS One 8:e64145.
  8. Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
  9. Levy, E. et al. (2013) Atherosclerosis 227:297.
  10. Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
  11. Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
  12. Kosenko, T. et al. (2013) J. Biol. Chem. 288:8279.
  13. Mayer, G. et al. (2008) J. Biol. Chem. 283:31791.
  14. Benjannet, S. et al. (2006) J. Biol. Chem. 281:30561.
  15. Benjannet, S. et al. (2010) J. Biol. Chem. 285:40965.
  16. Essalmani, R. et al. (2011) J. Biol. Chem. 286:4257.
  17. Han, B. et al. (2014) J. Lipid Res. 55:1505.

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)

Gene Symbol

PCSK9

UniProt

Q80W65

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

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Product Specific Notices for Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

For research use only

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