Recombinant Mouse RBP4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3476-LC
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse RBP4/Retinol-Binding Protein 4 protein
Glu19-Leu201, with a C-terminal Ile and 6-His tag
Glu19-Leu201, with a C-terminal Ile and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu19
Predicted Molecular Mass
22 kDa
SDS-PAGE
22 kDa, reducing conditions
Activity
Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.
The 50% binding concentration (BC50) is >0.5 µM, as measured under the described conditions.
The 50% binding concentration (BC50) is >0.5 µM, as measured under the described conditions.
Formulation, Preparation and Storage
3476-LC
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile 50 mM Tris, 10 mM CaCl2 and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: RBP4/Retinol-Binding Protein 4
Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol‑binding protein 4 (RBP4), also known as plasma retinol‑binding protein (1-3). A prototypic member of the lipocalin superfamily, RBP4 has a
beta‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4‑retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol‑RBP4‑TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol‑RBP4 complex. Defects in RBP4 cause retinol‑binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases.
References
- Zanotti, G. and R. Berni (2004) Vitamins and Hormones 69:271.
- Newcomer, M.E. and D.E. Ong (2000) Biochim. Biophys. Acta 1482:57.
- Yang, Q. et al. (2005) Nature 436:356.
Alternate Names
RetinolBinding Protein 4
Gene Symbol
RBP4
UniProt
Additional RBP4/Retinol-Binding Protein 4 Products
Product Documents for Recombinant Mouse RBP4 Protein, CF
Product Specific Notices for Recombinant Mouse RBP4 Protein, CF
For research use only
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