Recombinant Mouse Serpin F2/alpha 2-Antiplasmin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1239-PI

R&D Systems, part of Bio-Techne
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1239-PI-010
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Key Product Details

Source

NS0

Accession #

Q61247

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Serpin F2/alpha 2-Antiplasmin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Serpin F2/alpha 2-Antiplasmin protein
Val28-Lys491, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val28

Predicted Molecular Mass

54 kDa

SDS-PAGE

67 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <1.0 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1239-PI
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Serpin F2/alpha 2-Antiplasmin

Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28 to 39) and a mature chain (residues 40 to 491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).

References

  1. Menoud, P.-A. et al. (1996) J. Clin. Invest. 97:2478.
  2. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  3. Potempa, J. et al. (1988) Science. 241:699.
  4. Sumi, Y. et al. (1989) J. Biochem. 106:703.
 

Alternate Names

alpha 2-Antiplasmin

Entrez Gene IDs

5345 (Human); 18816 (Mouse)

Gene Symbol

SERPINF2

UniProt

Q61247

Additional Serpin F2/alpha 2-Antiplasmin Products

Product Documents for Recombinant Mouse Serpin F2/alpha 2-Antiplasmin Protein, CF

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Product Specific Notices for Recombinant Mouse Serpin F2/alpha 2-Antiplasmin Protein, CF

For research use only

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