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Recombinant Mouse SLAM/CD150 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4330-SL

R&D Systems, part of Bio-Techne
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4330-SL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLAM/CD150 protein
Thr25-Pro242, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

25 kDa

SDS-PAGE

41-55 kDa, reducing conditions

Activity

Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3.
The ED50 for this effect is 0.6-2.4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Reviewed Applications

Read 1 review rated 4 using 4330-SL in the following applications:

Formulation, Preparation and Storage

4330-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mature mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (3). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2-7). It is up‑regulated on activated B cells and CD4+ and CD8+ T cells, although it is down‑regulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages to Gram negative bacteria (8, 12-14). In humans, SLAM functions as a cellular entry receptor for measles virus (15, 16).

References

  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
  2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  3. Cocks, B.G. et al. (1995) Nature 376:260.
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
  5. Hahm, B. et al. (2004) Virology 323:292.
  6. Nanda, N. et al. (2005) Blood 106:3028.
  7. Kiel, M.J. et al. (2005) Cell 121:1109.
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  13. Jordan, M.A. et al. (2011) J. Immunol. 186:3953.
  14. Berger, S.B. et al. (2010) Nat. Immunol. 11:920.
  15. Tatsuo, H. et al. (2000) Nature 406:893.
  16. Hsu, E.C. et al. (2001) Virology 279:9.

Long Name

Signaling Lymphocytic Activation Molecule

Alternate Names

CD150, IPO-3, SLAMF1

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Gene Symbol

SLAMF1

UniProt

Additional SLAM/CD150 Products

Product Documents for Recombinant Mouse SLAM/CD150 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse SLAM/CD150 Protein, CF

For research use only

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