Recombinant Mouse Slit1 (aa 1126-1531) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9879-SL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q80TR4

Conjugate

Unconjugated

Applications

Bioactivity

View all Slit1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Slit1 protein
Ser1126-Ala1531, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser1126

Predicted Molecular Mass

45 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse Slit1, immobilized at 1.25-2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Mouse Slit1 (aa 1126-1531) Protein, CF

Recombinant Mouse Slit1 (aa 1126-1531) Protein SDS-PAGE

Recombinant Mouse Slit1 (aa 1126-1531) Protein SDS-PAGE

2μg/lane of Recombinant Mouse Slit1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60 - 68 kDa.

Formulation, Preparation and Storage

9879-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Slit1

Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1, 2). Like other mammalian family members, the 1531 amino acid (aa), ~200 kDa Slit1 contains a 33 aa signal sequence followed by 23 leucine-rich repeats (LRR, aa 34-900) and 9 EGF-like sequences (aa 930-1451) (2). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7 (aa 1163-1336), and a C-terminal cysteine-rich domain (cysteine knot; aa 1456-1531) (2). Heparin sulfates are required for interaction of Robo with Slit LRR domains (2, 3). The C-terminal fragment (aa 1126-1531) of mouse Slit 1 shares 96% and 99% aa identity with human and rat respectively. Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary locations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hippocampal and motor axons (1, 4-9). Deletion of either Slit1 or Slit2 has less effect than deletion of both, which allows axons to wander from tracts and inappropriately cross or recross the midline (4, 5, 7-9). In the injured spinal cord, presence of Slit1 along with Slit3 and Netrin-1 may be responsible for failure of axons to regenerate in the adult CNS (10). Slit1 also promotes dendrite growth and branching of cortical neurons indicating it may exert important influence on the final morphology of cortical neurons (11). Although Slit1 has mainly been found in the fetal and adult brain, it is also detected in the heart and kidney. The C-terminal cysteine knot, which may mediate interaction with other proteins, is absent in the rat brain splicing variant, Slit1 alpha (12).

References

  1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  2. Hohenester, E. (2008) Biochem. Soc. Trans. 36:251.
  3. Hussain, S-A. et al. (2006) J. Biol. Chem. 281:39693.
  4. Thompson, H. et al. (2006) Dev. Biol. 296:476.
  5. Plump, A. S. et al. (2002) Neuron 33:219.
  6. Cho, J. H. et al. (2007) J. Neurosci. 27:9094.
  7. Nguyen-Ba-Charvet, K. T. et al. (2002) J. Neurosci. 22:5473.
  8. Bagri, A. et al. (2002) Neuron 33:233.
  9. DiMeglio, T. et al. (2008) J. Neurosci. 28:6285.
  10. Wehrle, R. et al. (2005) Eur. J. Neurosci. 22:2134.
  11. Whitford, K. L. et al. (2002) Neuron 33:47.
  12. Tanno, T. et al. (2004) J. Biochem. 136:575.

Long Name

SLIT Homolog 1

Alternate Names

MEGF4, SLIL1

Entrez Gene IDs

6585 (Human); 20562 (Mouse)

Gene Symbol

SLIT1

UniProt

Q80TR4

Additional Slit1 Products

Product Documents for Recombinant Mouse Slit1 (aa 1126-1531) Protein, CF

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Product Specific Notices for Recombinant Mouse Slit1 (aa 1126-1531) Protein, CF

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