Recombinant Mouse SLITRK6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1812-SK

His-tag
R&D Systems, part of Bio-Techne
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1812-SK-050
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Key Product Details

Source

NS0

Accession #

Q8C110

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK6 protein
Met1-Ser607, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

66 kDa

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.

Scientific Data Images for Recombinant Mouse SLITRK6 Protein, CF

Recombinant Mouse SLITRK6 Protein SDS-PAGE

Recombinant Mouse SLITRK6 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse SLITRK6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-90 kDa.

Formulation, Preparation and Storage

1812-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: SLITRK6

SLITRK6 (Slit and Trk-like family member 6) is a type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region. Mature mouse SLITRK6 consists of a 587 amino acid (aa) extracellular domain (ECD) with 5 leucine rich repeats (LRR) flanked by N-and C-terminal LRR domains, followed by 6 more LRR flanked by another pair of N- and C-terminal LRR domains (1, 2). Within the ECD, mouse SLITRK6 shares 89% and 95% aa sequence identity with human and rat SLITRK6, respectively. SLITRK6 is strongly expressed in the auditory and vestibular sensory epithelia of the ear (3). Its expression in the inner ear promotes innervation and survival of sensory neurons (3, 4). In the brain, SLITRK6 expression is highly restricted in the thalamus, lateral geniculate nucleus, and absent in the cortex (1, 3). SLITRK6 can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (2, 5). Mice lacking SLITRK6 exhibit delayed retinal synaptogenesis and multiple auditory abnormalities as well as defective auditory sensory innervation, neuronal loss in the vestibular ganglia, and reduced cochlear expression of BDNF, NT-3, TrkB, and TrkC (3, 6, 7). In humans, nonsense mutations of SLITRK6 are associated with myopia and sensorial deafness (6).

References

  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  3. Katayama, K.-I. et al. (2009) PLoS ONE 4:e7786.
  4. Morlet, T. et al. (2014) Laryngoscope. 124:95.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Tekin, M. et al. (2013) J. Clin. Invest. 123:2094.
  7. Matsumoto, Y. et al. (2011) PLoS ONE 6:e16497.

Long Name

SLIT and NTRK-like Family, Member 6

Alternate Names

FLJ22774, MGC119595, MGC119596, MGC119597,4832410J21Rik, SLIT and NTRK-like family, member 6, SLIT and NTRK-like protein 6, slit and trk like gene 6

Entrez Gene IDs

84189 (Human); 239250 (Mouse); 290467 (Rat)

Gene Symbol

SLITRK6

UniProt

Q8C110

Additional SLITRK6 Products

Product Documents for Recombinant Mouse SLITRK6 Protein, CF

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Product Specific Notices for Recombinant Mouse SLITRK6 Protein, CF

For research use only

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