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Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9406-SO

R&D Systems, part of Bio-Techne
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9406-SO-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Stanniocalcin 2/STC-2 protein
Thr25-Arg296, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

31 kDa

SDS-PAGE

33-44 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 75-450 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Images for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

Recombinant Mouse Stanniocalcin 2/STC-2 Protein Bioactivity

Recombinant Mouse Stanniocalcin 2/STC-2 Protein Bioactivity

Recombinant Mouse Stanniocalcin 2/STC-2 (Catalog # 9406-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 75-450 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Formulation, Preparation and Storage

9406-SO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stanniocalcin 2/STC-2

Mouse STC-2 (Stanniocalcin-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein. It is first discovered from corpuscles of stannous, an endocrine gland unique to bony fish (1-3). The 272 amino acid (aa) mature mouse STC-2 has an 86% homology with the human form. STC-2 has been described as having a role in human adult height and is involved in the GIANT consortium (4, 5). STC-2 is expressed in a wide variety of tissues. It regulates calcium and phosphate homeostasis (1-3), enhances mesenchymal stem cell survival (6), promotes cell proliferation in cervical cancer (7), suppresses breast cancer cell migration and invasion (8), promotes osteoblast differentiation (9), and inhibits longitudinal bone growth directly at the growth plate (10). It is also a biomarker for cervical and lung cancers (11, 12).Fform

References

  1. Wagner, G.F. et al. (1995) Proc. Natl. Acad. Sci. 92:1871.
  2. Zhang, J. et al. (1998) Protein Expression and Purification. 12:390.
  3. Takei, Y. et al. (2012) Bone. 50:998.
  4. Marouli, E. et al. (2017) Nature 542:186.
  5. Holmes, D. (2017) Nature Reviews Endocrinology. 13:190.
  6. Kim PH, et al. (2015) BMB Rep. 48:702.
  7. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
  8. Hou J, et al. (2015) PLoS One. 10:e0122179.
  9. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
  10. Wu S, et al. (2006) J Biol Chem. 281:5120.
  11. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
  12. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

Alternate Names

STC-2, STC2, STCRP

Entrez Gene IDs

8614 (Human); 20856 (Mouse)

Gene Symbol

STC2

UniProt

Additional Stanniocalcin 2/STC-2 Products

Product Documents for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

For research use only

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