Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9406-SO
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Stanniocalcin 2/STC-2 protein
Thr25-Arg296, with a C-terminal 6-His tag
Thr25-Arg296, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr25
Predicted Molecular Mass
31 kDa
SDS-PAGE
33-44 kDa, reducing conditions
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 75-450 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
The ED50 for this effect is 75-450 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Scientific Data Images for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF
Recombinant Mouse Stanniocalcin 2/STC-2 Protein Bioactivity
Recombinant Mouse Stanniocalcin 2/STC-2 (Catalog # 9406-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 75-450 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).Formulation, Preparation and Storage
9406-SO
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 500 μg/mL in PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Stanniocalcin 2/STC-2
References
- Wagner, G.F. et al. (1995) Proc. Natl. Acad. Sci. 92:1871.
- Zhang, J. et al. (1998) Protein Expression and Purification. 12:390.
- Takei, Y. et al. (2012) Bone. 50:998.
- Marouli, E. et al. (2017) Nature 542:186.
- Holmes, D. (2017) Nature Reviews Endocrinology. 13:190.
- Kim PH, et al. (2015) BMB Rep. 48:702.
- Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
- Hou J, et al. (2015) PLoS One. 10:e0122179.
- Zhou J, et al. (2016) Mol Med Rep. 14:5653.
- Wu S, et al. (2006) J Biol Chem. 281:5120.
- Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
- Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.
Alternate Names
STC-2, STC2, STCRP
Gene Symbol
STC2
UniProt
Additional Stanniocalcin 2/STC-2 Products
Product Documents for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF
Product Specific Notices for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF
For research use only
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