Recombinant Mouse Syndecan-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3190-SD

R&D Systems, part of Bio-Techne
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3190-SD-050
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Key Product Details

Source

NS0

Accession #

P18828

Conjugate

Unconjugated

Applications

Bioactivity

View all Syndecan-1/CD138 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Syndecan-1/CD138 protein
Gln18-Glu252, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln18 predicted

Predicted Molecular Mass

25.3 kDa

SDS-PAGE

60-80 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin.
When 5 x 104 cells/well are added to mouse Syndecan-1 and Recombinant Human Fibronectin (0.5 μg/mL, Catalog # 4305-FNB) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 2.5‑10 μg/mL.

Formulation, Preparation and Storage

3190-SD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-1/CD138

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the Syndecan family of Type 1 transmembrane proteins (1, 2). The four Syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 22 aa signal sequence, a 233 aa extracellular domain (ECD) that includes three closely spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 21 aa TM segment, and a 35 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site (4). The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Mouse Syndecan-1 ECD shares 70% and 87% aa identity with the ECD of human and rat Syndecan-1, respectively. Alternative splicing in mouse generates an isoform with an internal deletion of 44 aa from the ECD (5). Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (6, 7). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (6 - 10). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (7, 9, 11). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (12 - 14). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (14, 15). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (6).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
  3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  4. Saunders, S. et al. (1989) J. Cell Biol. 108:1547.
  5. Romaris, M. et al. (1999) J. Biol. Chem. 274:18667.
  6. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
  7. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
  8. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
  9. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
  10. Vanhoutte, D. et al. (2007) Circulation 115:475.
  11. Li, Q. et al. (2002) Cell 111:635.
  12. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
  13. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
  14. Derksen, P.W.B. et al. (2002) Blood 99:1405.
  15. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Alternate Names

CD138, SDC1, Syndecan1

Entrez Gene IDs

6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

Gene Symbol

SDC1

UniProt

P18828

Additional Syndecan-1/CD138 Products

Product Documents for Recombinant Mouse Syndecan-1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Syndecan-1 Protein, CF

For research use only

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