Recombinant Mouse Testisin/Prss21 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6820-SE

R&D Systems, part of Bio-Techne
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6820-SE-010
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Key Product Details

Source

NS0

Accession #

Q9JHJ7

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Testisin/Prss21 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Testisin/Prss21 protein
Leu22-Asn298, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

33 kDa

SDS-PAGE

46-50 kDa, reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >330 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6820-SE
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2 and Brij-35.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Testisin/Prss21

Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase‑4 (1). It is predicted to have substrate specificity similar to that of trypsin (2). Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils (3). Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility (4, 5). Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor (6). Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions.

References

  1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648.
  2. Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63.
  3. Inoue, M. et al. (1998) Biochem. Biophys.  Res. Comm. 252:307.
  4. Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921.
  5. Kawano, N. et al. (2010) Biol. Reprod. 83:359.
  6. Honda, A. et al. (2002) J. Biol. Chem. 277:16976.

Alternate Names

Prss21, TEST1

Entrez Gene IDs

10942 (Human); 57256 (Mouse)

Gene Symbol

PRSS21

UniProt

Q9JHJ7

Additional Testisin/Prss21 Products

Product Documents for Recombinant Mouse Testisin/Prss21 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Testisin/Prss21 Protein, CF

For research use only

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