Recombinant Mouse TFPI Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2975-PI

R&D Systems, part of Bio-Techne
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2975-PI-010
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Key Product Details

Source

NS0

Accession #

O54819

Conjugate

Unconjugated

Applications

Inhibition Activity

View all TFPI Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TFPI protein
Leu29-Lys289, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

31 kDa

SDS-PAGE

41 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <1 nM, as measured under the described conditions.

Formulation, Preparation and Storage

2975-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and Brij-35.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI

Tissue Factor Pathway Inhibitor (TFPI), also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region (2). The first K domain (residues 50 to 100) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 121 to 171) inhibits factor Xa. The third K domain (residues 225 to 275) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in the binding of TFPI to cell surfaces (2). The purified recombinant mouse TFPI ends at residue 289 and does not contain the last 17 residues (residues 290 to 306) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # http://www.rndsystems.com/product_results.aspx?k=2338-SE">2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # http://www.rndsystems.com/product_results.aspx?k=2339-PA">2339-PA).

References

  1. Bai, H. et al. (2005) Thromb Haemost. 93:1055.
  2. Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
  3. Mine, S. et al. (2002) Biochemistry 41:78.

Long Name

Tissue Factor Pathway Inhibitor

Alternate Names

EPI, LACI

Entrez Gene IDs

7035 (Human); 21788 (Mouse)

Gene Symbol

TFPI

UniProt

O54819

Additional TFPI Products

Product Documents for Recombinant Mouse TFPI Protein, CF

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Product Specific Notices for Recombinant Mouse TFPI Protein, CF

For research use only

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