Recombinant Mouse TfR (Transferrin R) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9706-TR

R&D Systems, part of Bio-Techne
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9706-TR-050
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Key Product Details

Source

CHO

Accession #

Q62351

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TfR (Transferrin R) Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TfR (Transferrin R) protein
Cys89-Phe763, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

77 kDa

SDS-PAGE

75-86 kDa (major) and 133-157 kDa (minor), under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse TfR (Transferrin R) (Catalog # 9706-TR) is immobilized at 5 µg/mL (100 µL/well), Biotinylated Mouse Holo-Transferrin binds with an ED50 of 20.0-160 ng/mL.

Scientific Data Images for Recombinant Mouse TfR (Transferrin R) Protein, CF

Recombinant Mouse TfR (Transferrin R) Protein Binding Activity

Recombinant Mouse TfR (Transferrin R) Protein Binding Activity

When Recombinant Mouse TfR (Catalog # 9706-TR) is coated at 5 µg/mL (100 µg/mL), Biotinylated Mouse Holo-Transferrin binds with an ED50 of 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

9706-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR (Transferrin R)

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type II transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1-4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome (2, 5). The mouse TfR cDNA encodes 763 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 675 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site (5). Mouse TfR ECD shares 76% and 91% aa identity with human and rat, respectively. A cleaved and truncated 85 kDa soluble form of TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR (3). sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency (3). Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production (3). Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue (2, 4). Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis (2, 4). The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling (2, 5). TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses (2, 4, 6).

References

  1. Stearne P.A. et al. (1985) J. Immunol. 134:3474.
  2. Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
  3. Skikne, B.S. (2008) Am. J. Hematol. 83:872.
  4. Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
  5. Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
  6. Radoshitzky, S.R. et al. (2007) Nature 446:92.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Entrez Gene IDs

7037 (Human); 22042 (Mouse); 64678 (Rat)

Gene Symbol

TFRC

UniProt

Q62351

Additional TfR (Transferrin R) Products

Product Documents for Recombinant Mouse TfR (Transferrin R) Protein, CF

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