Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 587-RI

This item is in process of being discontinued.
R&D Systems, part of Bio-Techne
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587-RI-050
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

BAA05023

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TGF-beta RI/ALK-5 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TGF-beta RI/ALK-5 protein
Mouse TGF-beta RI
(Ala21-Glu121) &
(Thr22-Glu121)
Accession # BAA05023		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21 & Thr22

Predicted Molecular Mass

37.5 kDa (monomer)

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Endoglin/CD105 Fc Chimera (Catalog # 6440-EN) or Recombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.25-1.25 μg/mL.

Formulation, Preparation and Storage

587-RI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

TGF-beta  RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, mouse TGF-beta RI shares 98% and 90% amino acid sequence identity with rat and human TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).

References

  1. Wrana, J.L. et al. (1994) Nature 370:341.
  2. Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
  3. Huse, M. et al. (1999) Cell 96:425.
  4. Marcías-Silva, M. et al. (1996) Cell 87:1215.
  5. Zhang, Y. et al. (1996) Nature 383:168.
  6. Huse, M. et al. (2001) Mol. Cell 8:671.
  7. Larsson, J. et al. (2001) EMBO J. 20:1663.
  8. James, J.M. et al. (2013) Development 140:3903.
  9. Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

BAA05023

Additional TGF-beta RI/ALK-5 Products

Product Documents for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

For research use only

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