Recombinant Mouse Tie-2 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 762-T2
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Tie-2 protein
| Mouse Tie-2 (Ala23 - Lys744) Accession # CAA47857 |
DIEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala23
Predicted Molecular Mass
107.2 kDa (monomer)
SDS-PAGE
125-135 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhAngiopoietin-2 with a linear range of 2-50 ng/mL.
Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhAngiopoietin-2 with a linear range of 2-50 ng/mL.
Formulation, Preparation and Storage
762-T2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
References
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Entrez Gene IDs
Gene Symbol
TEK
UniProt
Additional Tie-2 Products
Product Documents for Recombinant Mouse Tie-2 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse Tie-2 Fc Chimera Protein, CF
For research use only
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