Recombinant Mouse TMEM119 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10434-TM

R&D Systems, part of Bio-Techne
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10434-TM-050
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Key Product Details

Source

CHO

Accession #

Q8R138.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TMEM119 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TMEM119 protein
Mouse TMEM119
(Tyr21-Met91)
Accession # Q8R138.1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr21

Predicted Molecular Mass

35 kDa

SDS-PAGE

37-60 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse TMEM119 Fc Chimera (Catalog # 10434-TM), immobilized at 1.25 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Mouse TMEM119 Fc Chimera Protein, CF

Recombinant Mouse TMEM119 Fc Chimera Protein SDS-PAGE

Recombinant Mouse TMEM119 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse TMEM119 Fc Chimera (Catalog # 10434-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-60 kDa and 70-120 kDa, respectively.

Formulation, Preparation and Storage

10434-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM119

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3). Mature mouse TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain. Within the ECD, mouse TMEM119 shares 78% and 87% aa sequence identity with human and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).

References

  1. Jiang, Z.H. et al. (2017) Exp. Mol. Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Satoh, J. et al. (2016) Neuropathology 36:39.
  4. Kanamoto, T. et al. (2009) BMC Dev. Biol. 9:70.

Long Name

Transmembrane Protein 119

Alternate Names

OBIF

Entrez Gene IDs

338773 (Human); 231633 (Mouse)

Gene Symbol

TMEM119

UniProt

Q8R138.1

Additional TMEM119 Products

Product Documents for Recombinant Mouse TMEM119 Fc Chimera Protein, CF

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