Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9707-R2

R&D Systems, part of Bio-Techne
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9707-R2-050
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Key Product Details

Source

NS0

Accession #

P25119

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TNF RII/TNFRSF1B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TNF RII/TNFRSF1B protein
Mouse TNF RII/TNFRSF1B
(Val23-Gly258)
Accession # P25119		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23

Predicted Molecular Mass

52 kDa

SDS-PAGE

66-76 kDa, reducing conditions

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect is 3-15 ng/mL

Scientific Data Images for Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein Binding Activity

Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein Binding Activity

Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 9707-R2) inhibits Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT) mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3-15 ng/mL.

Formulation, Preparation and Storage

9707-R2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta (1, 2). These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A (3). In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions (4). Mature mouse TNF RII contains an extracellular domain (ECD, aa 23 - 258) containing ten disulfide bonds, a transmembrane domain (aa 259-288), and a cytoplasmic domain (aa 289-474). Within the ECD, the mouse TNF RII shares 57% and 84% aa sequence identity to human and rat TNF RII. TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (5-7). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (5, 8). However, under certain conditions, TNF RII signaling can induce apoptosis (5). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (9).

References

  1. Smith, C.A. et al. (1990) Science 248: 1019.
  2. Goodwin R.G. et al. (1991) Mol. Cell. Biol. 11:3020.
  3. Kohno, T. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8331.
  4. Yamada, Y. et al. (2000) Burns 26:239.
  5. Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
  6. Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
  7. Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
  8. Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
  9. Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

UniProt

P25119

Additional TNF RII/TNFRSF1B Products

Product Documents for Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

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