Recombinant Mouse TREML1/TLT-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2424-TL

R&D Systems, part of Bio-Techne
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2424-TL-050
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Key Product Details

Source

NS0

Accession #

AAL85488

Conjugate

Unconjugated

Applications

Bioactivity

View all TREML1/TLT-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TREML1/TLT-1 protein
Gly18-Cys178 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly18

Predicted Molecular Mass

18.3 kDa

SDS-PAGE

21-23 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of thrombin-activated human platelets.

When 5 x 106 cells per well are added to rmTLT-1 coated plates (2 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 1 hour at 37 °C.

Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2424-TL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML1/TLT-1

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT-1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain (SwissProt #: Q8K558). The ECD contains an Ig-like V-type domain (aa 21 - 122), and the cytoplasmic region contains a proline-rich region (aa 245 - 275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha-granules in resting platelets and on the cell surface of platelets activated by thrombin (1 - 3). The receptor’s expression is upregulated dramatically upon platelet activation (2). Antibodies to single-chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilonRI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

References

  1. Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
  2. Washington, A.V. et al. (2004) Blood 104:1042.
  3. Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
  4. Washington, A.V. et al. (2002) Blood 100:3822.
  5. Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Alternate Names

TLT-1, TLT1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Gene Symbol

TREML1

UniProt

AAL85488

Additional TREML1/TLT-1 Products

Product Documents for Recombinant Mouse TREML1/TLT-1 Protein, CF

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Product Specific Notices for Recombinant Mouse TREML1/TLT-1 Protein, CF

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