Recombinant Mouse TRH-degrading Ectoenzyme/TRHDE Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2985-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse TRH-degrading Ectoenzyme/TRHDE protein
Arg64-His1025, with an N-terminal 6-His tag
Arg64-His1025, with an N-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
112 kDa
SDS-PAGE
131 kDa, reducing conditions
Activity
Measured by its ability to cleave the pyroGlu-His bond in a fluorogenic peptide substrate, pyroGlu-His-Pro-7-amido-4-methylcoumarin (pEHP-AMC). The resulting HP-AMC was cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE).
The specific activity is >130 pmol/min/µg, as measured under the described conditions.
The specific activity is >130 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2985-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: TRH-degrading Ectoenzyme/TRHDE
TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human.
References
- Schmitmeier, S. et al. (2002) Eur. J. Biochem. 269:1278.
- Kelly, J.A. et al. (2005) Biochem. J. 389:569.
Long Name
Thyrotropin Releasing Hormone-degrading Ectoenzyme
Alternate Names
PAP-II, PGPEP2, Thyroliberinase, TRH-DE, TRHDE, TRHdegrading Ectoenzyme
Gene Symbol
TRHDE
UniProt
Additional TRH-degrading Ectoenzyme/TRHDE Products
Product Documents for Recombinant Mouse TRH-degrading Ectoenzyme/TRHDE Protein, CF
Product Specific Notices for Recombinant Mouse TRH-degrading Ectoenzyme/TRHDE Protein, CF
For research use only
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